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    COLLOQUIUM OF THE LABORATORY FOR COMPUTER DESIGN OF MATERIALS
        Institute for Computational Sciences and Informatics
	                      (CSI 898-Sec 001) 

       Protein solution and crystallization dynamics: experimental 
                     and computational techniques

                            Sridhar Gorti
              Naval Research Laboratory,Washington, DC 
  
    Measurements of lysozyme diffusion within the "depletion zone" of 
the (110) face of lysozyme crystals are reported.  The measurements 
were performed using the technique of microscope light scattering (MLS), 
capable of measuring small sampling areas (~2 µm2).  Measurements 
within such small areas allowed for the determination of intensity 
correlation functions at precise locations (at ~10 µm intervals) 
adjacent to the (110) crystal face.  Using well-established methods 
in the analyses of intensity correlation functions, the diffusion 
coefficients of lysozyme molecules adjacent to the (110) crystal 
face and in solution were obtained.  The diffusion coefficients 
were observed to decrease as the surface of the crystal was approached.  
This decrease is shown to occur, in theory and model calculations, as 
a result of the depletion of lysozyme molecules, whose diffusion 
coefficients are ~8 x 10-7 cm2/s at 23 °C.

	                  Monday , Spetember 20 1999
                                  4:30 pm
                         Room 206, Science & Tech. I

Refreshments will be served.
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